Regulatory

Part:BBa_K389050:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-09-28)

Tightly controlled lac operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we needed a tightly controlled inducible promoter, so a lacIq promoter + lacI repressor is added to a lac operon

Source

parts.igem

References